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Journal / Bioengineering / Poly-transfection을 이용한 세포 상태 식별 회로의 신속한…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Poly-transfection을 이용한 세포 상태 식별 회로의 신속한 개발
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Poly-transfection을 이용한 세포 상태 식별 회로의 신속한 개발

Article DOI: 10.3791/64793-v • 09:21 min • February 24th, 2023
February 24th, 2023 •
1, 1,2, 3, 1,3
1Department of Biological Engineering, Massachusetts Institute of Technology, 2School of Biological Engineering, University of British Columbia, 3Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology

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복잡한 유전 회로는 설계, 테스트 및 최적화에 시간이 많이 걸립니다. 이 과정을 촉진하기 위해 포유류 세포는 단일 웰에서 회로 구성 요소의 여러 화학량론을 테스트할 수 있는 방식으로 형질감염됩니다. 이 프로토콜은 실험 계획, transfection 및 데이터 분석을 위한 단계를 간략하게 설명합니다.

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생명공학 합성생물학 유전회로 세포분류기 고처리량 최적화 유전자회로 신속프로토타이핑 형질감염 유세포 분석
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