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Journal / Immunology and Infection / Anrikning av naturliga och rekombinanta…
JoVE Journal
Immunology and Infection

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Anrikning av naturliga och rekombinanta extracellulära vesiklar av mykobakterier
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Anrikning av naturliga och rekombinanta extracellulära vesiklar av mykobakterier

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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Detta protokoll beskriver anrikningen av inhemska mykobakteriella extracellulära vesiklar (mEV) från axeniska kulturer av Mycobacterium smegmatis (Msm) och hur mCherry (en röd fluorescerande reporter)-innehållande rekombinanta MsmEVs kan designas och anrikas. Slutligen verifieras det nya tillvägagångssättet genom anrikning av MsmEVs som innehåller EsxA-proteinet Mycobacterium tuberculosis.

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Immunologi och infektion utgåva 202 Extracellulära vesiklar rekombinanta vesiklar Esat-6 mykobakterier ExsA mCherry Cfp-29 bakterier
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