Photoactivation Chemistry
Photoactivation Chemistry - Related Videos
11:20
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
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Cited by 1 •
2017
In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol.
05:02
Bimolecular Fluorescence Complementation-Coupled Photoactivated Localization Microscopy
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2025
This video describes BiFC-PALM ― a combination of photoactivated localization microscopy and bimolecular fluorescence complementation ― to assess protein-protein interactions. BiFC involves the fusion of two fluorescent protein fragments with two interacting proteins of interest. When the two proteins interact, the fragments are brought into proximity, which allows the two parts to come together and reconstitute a functional fluorescent protein. The fluorescence of a single fluorophore is...
08:42
Coordination Chemistry Complexes
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2023
Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry Transition metals are found everywhere from vitamin supplements to electroplating baths. Transition metals also make up the pigments in many paints and compose all minerals. Typically, transition metals are found in the cationic form since they readily oxidize, or lose electrons, and are surrounded by electron donors called ligands. These ligands do not form ionic or covalent bonds with the metal center,...
12:31
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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Cited by 14 •
2012
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
09:14
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
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Cited by 8 •
2016
This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.
FAQs
Photon absorption can move a molecule into an excited state with enough altered reactivity to initiate several outcomes. Depending on the molecular design, the excited state may promote bond cleavage, release a previously caged compound, generate a reactive intermediate, or activate a photoinitiator. These distinct pathways let researchers connect the same optical input to different chemical functions.
Wavelength and exposure conditions determine whether the intended molecular component responds and how precisely activation can be controlled. Selecting suitable optical conditions helps restrict activity to a chosen location or time, which is especially important when chemical changes must occur within a defined cellular environment, material region, or patterned bioengineering construct.
These components provide different ways to translate light into a useful chemical change. A caged compound can remain inactive until light releases it, a reactive intermediate can drive subsequent chemistry, and a photoinitiator can start a reaction such as hydrogel formation. Choosing among them allows the optical trigger to match the desired molecular or material response.
Spatial control comes from directing light to a selected region, while temporal control comes from choosing when exposure occurs. Linking these optical controls to chemical activation allows researchers to release compounds, pattern biomolecules, form material structures, or regulate cellular environments at defined places and times. This capability is valuable for studying dynamic biological processes.
A design should identify the desired chemical outcome, the light-responsive component, and the wavelength and exposure conditions needed to produce it. Researchers then align that response with the target system, such as a caged compound for release, a photoinitiator for hydrogel formation, or a patterning strategy for biomolecules. The goal is controlled activity rather than untargeted chemical change.
Photoactivation chemistry supports several bioengineering applications described in the source material. It can enable spatially and temporally controlled drug release, biomolecule patterning, hydrogel formation, and regulation of cellular environments. These applications use optical inputs to create localized or timed chemical changes, helping researchers design responsive materials and investigate biological processes that change over time.