Syringe Pump Calibration
Syringe Pump Calibration - Related Videos
09:08
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Cited by 3 •
2018
Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.
07:43
Calibration Curves
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Calibration curves are used to understand the instrumental response to an analyte and predict the concentration in an unknown sample. Generally, a set of standard samples are made at various concentrations with a range than includes the unknown of interest and the instrumental response at each concentration is recorded. For more accuracy and to understand the error, the response at each concentration can be repeated so an error...
01:11
Glassware Calibration
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2024
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes. Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
10:22
High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
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Cited by 1 •
2009
A novel microfluidic system has been developed using the phenomenon of passive pumping and a user controlled fluid delivery system. This microfluidic system has the potential to be used in a wide variety of biological applications given its low cost, ease of use, volumetric precision, high speed, repeatability and automation.
01:30
ATP Driven Pumps III: V-type Pumps
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2023
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient. The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
FAQs
Syringe size determines how much fluid corresponds to a given amount of plunger travel, so the programmed setting may not produce the same output across syringe configurations. Plunger movement also contributes directly to delivered volume and flow rate. Evaluating these variables during calibration helps identify systematic differences between commanded delivery and actual output, improving reproducibility in quantitative experiments.
Flow resistance can alter how readily fluid leaves the syringe, while operating conditions may change the relationship between programmed settings and delivered output. These effects make calibration dependent on more than the nominal pump command alone. Including relevant resistance and operating conditions in verification helps reveal performance deviations that could otherwise affect dosing, flow control, or experimental comparisons.
The comparison shows whether the pump delivers the programmed volume and flow rate or produces a systematic error. A consistent difference indicates that the instrument or its operating configuration may require corrective adjustment, whereas a close match supports confidence in the programmed setting. Recording this comparison also creates a performance record for later verification and interpretation of results.
Begin with the syringe and operating configuration used for the experiment, then program the intended volume or flow rate and measure the resulting output. Compare the measured value with the commanded value, identify any discrepancy, and make corrective adjustments when needed. Documenting the comparison and adjustment provides a record that supports reproducible operation and subsequent performance checks.
Verification is especially important when experiments depend on small, quantitative fluid deliveries or controlled flow. Bioengineering workflows such as cell culture, microfluidics, drug-delivery studies, and tissue engineering can be sensitive to dosing or flow deviations. Regular checks help determine whether the pump remains suitable for the intended experiment and can improve confidence in measurements collected over time.
Calibration records connect programmed settings with measured pump performance and any corrective adjustments made. This documentation helps researchers assess whether an unexpected experimental result might coincide with delivery error rather than the biological system itself. It also supports comparisons between experiments by showing how instrument performance was verified, strengthening data quality and confidence in quantitative conclusions.