Vascularized Tissue Equivalents
Vascularized Tissue Equivalents - Related Videos
09:04
Generation of Self-assembled Vascularized Human Skin Equivalents
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Cited by 11 •
2021
The goal of this protocol is to describe the generation and volumetric analysis of vascularized human skin equivalents using accessible and simple techniques for long term culture. To the extent possible, the rationale for steps is described to allow researchers the ability to customize based on their research needs.
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
08:22
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
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Cited by 37 •
2017
We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.
08:43
Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
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Cited by 14 •
2018
The goal of the protocol is to build an inflammatory human gingiva model in vitro. This tissue model co-cultivates three types of human cells, HaCaT keratinocytes, gingival fibroblasts, and THP-1 macrophages, under three-dimensional conditions. This model can be applied to investigating periodontal diseases, such as gingivitis and periodontitis.
09:23
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
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Cited by 40 •
2015
In this work, we present a technique for the rapid fabrication of living vascular tissues by direct culturing of collagen, smooth muscle cells and endothelial cells. In addition, a new protocol for the mechanical characterization of engineered vascular tissues is described.
FAQs
As engineered tissues become thicker, cells located away from the surrounding environment may receive less oxygen and fewer nutrients. A vessel-like network addresses this transport limitation, helping maintain cell survival and supporting tissue function throughout the construct. This makes vascularized models more suitable than simpler systems for studying thicker tissues and processes that depend on sustained cellular viability.
Living cells provide the functional tissue component, while biomaterials create the three-dimensional setting in which cells can organize. Vascular-forming cues encourage endothelial cells to arrange into vessel-like structures, and the resulting organization can support integration with surrounding tissue. Their combined use links tissue function with vascular development rather than treating each feature as an isolated component.
The main advantage is improved support for cells within thicker three-dimensional constructs. By promoting nutrient and oxygen transport, vascularized versions can improve cell survival and produce responses that are more physiologically relevant than those of nonvascularized models. This distinction is important when researchers examine tissue development, disease behavior, drug response, or repair in conditions that require viable functional tissue.
A foundational workflow combines living tissue-specific cells with a biomaterial and vascular-forming cues. The system is then designed to promote endothelial organization and vessel formation, followed by consideration of how the construct integrates with surrounding tissue. These stages establish both the functional tissue component and the vascular feature needed to improve transport and cellular support.
Researchers may select these constructs when a model must represent tissue development, disease, drug response, or repair more realistically than a nonvascularized system. Their improved transport and cell survival are especially relevant to three-dimensional studies in which tissue thickness limits cellular support. The approach also contributes to research on regenerative medicine, transplantation, and predictive in vitro platforms.
Improved vascular support can increase cell survival and help tissue constructs behave more physiologically, strengthening their value as experimental models. In bioengineering, this can improve studies of how tissues develop, respond to disease or drugs, and participate in repair. The same features support longer-term goals involving regenerative medicine, transplantation research, and more predictive in vitro testing.