Fiber Stretching
Fiber Stretching - Related Videos
09:41
Stretching Micropatterned Cells on a PDMS Membrane
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Cited by 6 •
2014
This manuscript presents a technique to apply or release forces on adherent cells or tissues using unidirectional stretching.
08:28
Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
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Cited by 6 •
2017
A protocol for the microfluidic spinning and microstructure characterization of regenerated silk fibroin monofilament is presented.
07:42
Live Cell Imaging during Mechanical Stretch
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Cited by 12 •
2015
A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.
11:57
Fabricating Metamaterials Using the Fiber Drawing Method
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Cited by 23 •
2012
Metamaterials at terahertz frequencies offer unique opportunities, but are challenging to fabricate in bulk. We adapt the fabrication procedure for microstructured polymer optical fibers to inexpensively fabricate metamaterials potentially on an industrial scale. We produce polymethylmethacrylate fibers containing ~10 μm diameter indium wires separated by ~100 μm, which exhibit a terahertz plasmonic response.
11:46
Axon Stretch Growth: The Mechanotransduction of Neuronal Growth
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Cited by 30 •
2011
A unique tissue engineering method was developed to elongate numerous nerve fibers in culture by recapitulating axon stretch growth; a form of nervous system growth whereby nerves elongate in conjunction with growth of the enlarging body.
FAQs
Cells receive thymidine analogs in sequence, with each label marking DNA synthesized during a different labeling interval. After lysis and spreading, adjacent labeled segments along individual fibers preserve the temporal order of synthesis. Fluorescence patterns therefore convert replication history into measurable tracks, allowing investigators to distinguish and quantify replication activity at the level of single molecules.
DNA fiber analysis can quantify replication fork progression, origin firing, and fork symmetry on individual DNA molecules. These measurements provide a direct view of how replication proceeds rather than only an overall cellular average. Comparing the labeled patterns helps investigators identify differences in replication behavior and examine how experimental conditions affect genome duplication.
The method allows researchers to measure replication fork progression, origin firing, and fork symmetry under defined experimental conditions. Changes in these measurements can reveal altered replication behavior associated with replication stress. Because the assay examines individual DNA molecules, it can help identify replication defects relevant to genome instability, disease mechanisms, or responses to experimental treatments.
A typical workflow begins by sequentially labeling cells with thymidine analogs. Researchers then lyse the cells and spread the released DNA on a surface so the fibers become extended. Fluorescence microscopy is used afterward to visualize the labeled replication tracks along individual molecules, which are then analyzed for replication-related measurements.
Individual-molecule analysis connects visible fluorescence patterns with specific replication events along single DNA fibers. This perspective supports measurements of fork progression, origin firing, and fork symmetry that might be obscured when replication is assessed only across a whole cell population. The resulting data are useful for detecting replication defects and evaluating experimental responses.
Researchers can apply this approach when they need to examine DNA replication defects, genome instability, disease mechanisms, or cellular responses to experimental treatments. Its value comes from linking molecular activity to measurable replication tracks on individual fibers. In biology, that single-molecule context helps characterize how replication is organized and how it changes under different conditions.