Mouse Pancreas Dissection
Mouse Pancreas Dissection - Related Videos
08:21
Dissection of the Mouse Pancreas for Histological Analysis and Metabolic Profiling
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Cited by 14 •
2017
This video article provides a detailed demonstration of the procedures required to successfully remove the pancreas from a mouse by dissection for histological analysis and metabolic profiling.
08:35
In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors
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Cited by 26 •
2014
The three-dimensional culture method described in this protocol recapitulates pancreas development from dispersed embryonic mouse pancreas progenitors, including their substantial expansion, differentiation and morphogenesis into a branched organ. This method is amenable to imaging, functional interference and manipulation of the niche.
13:19
Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique
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Cited by 6 •
2017
Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 19 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
06:36
Primary Acinar Cell Isolation: A Method to Isolate Acinar Cells from Mouse Pancreas
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2023
This video describes the technique used for isolating pancreatic acinar cells--exocrine cells--containing a variety of digestive enzymes. Primary acinar cells are useful in the ex vivo study of acinar-to-ductal metaplasia (ADM)--an early event in developing pancreatic cancer.
FAQs
Anatomical relationships provide the landmarks needed to recognize pancreatic tissue within the abdominal cavity. They also help guide careful separation, because the tissue must be removed or sampled while minimizing damage to surrounding structures. Accurate identification is important for obtaining the intended organ material and for interpreting later observations of pancreatic structure and function.
Careful handling preserves the tissue for the type of examination that follows and reduces unwanted interference with the sample. This matters because isolated pancreatic tissue may undergo macroscopic examination, histology, or molecular and cellular analyses. A cleaner, less damaged sample supports more reliable evaluation of anatomical features, tissue organization, and experimental changes.
The procedure provides access to pancreatic tissue for investigating both digestive and endocrine functions. Examination can connect visible organ structure with tissue-level or molecular findings, while comparisons among experimental samples can reveal disease-related changes or responses to an intervention. Its value therefore extends beyond anatomy to the biological roles performed by the pancreas.
The workflow begins by exposing the abdominal cavity and locating the pancreas using its relationship to nearby organs. Researchers then separate the organ carefully and either remove the tissue or collect a sample while limiting damage and contamination. The material can subsequently be examined macroscopically or processed for histological, molecular, or cellular analysis.
The analytical approach should match the biological question. Macroscopic examination is suited to observing the isolated tissue directly, whereas histology evaluates tissue organization after processing. Molecular and cellular analyses provide other ways to investigate pancreatic characteristics. Selecting among these options determines whether the study emphasizes anatomy, tissue structure, cellular properties, or molecular responses.
This technique is useful when investigators need pancreatic tissue from a well-established mammalian model to examine anatomy, digestive or endocrine functions, disease-related changes, or experimental responses. Because the collected material can support several analytical approaches, one dissection workflow can contribute to structural studies as well as molecular and cellular investigations of pancreatic biology.