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JoVE Journal
Biology

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In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors
 

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

Article DOI: 10.3791/51725
July 19th, 2014

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Summary July 19th, 2014

The three-dimensional culture method described in this protocol recapitulates pancreas development from dispersed embryonic mouse pancreas progenitors, including their substantial expansion, differentiation and morphogenesis into a branched organ. This method is amenable to imaging, functional interference and manipulation of the niche.

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