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08:35 min
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July 19, 2014
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Summary
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The three-dimensional culture method described in this protocol recapitulates pancreas development from dispersed embryonic mouse pancreas progenitors, including their substantial expansion, differentiation and morphogenesis into a branched organ. This method is amenable to imaging, functional interference and manipulation of the niche.
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Cite this Article
Greggio, C., De Franceschi, F., Figueiredo-Larsen, M., Grapin-Botton, A. In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors. J. Vis. Exp. (89), e51725, doi:10.3791/51725 (2014).
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