Petri Dish
Petri Dish - Related Videos
03:57
Microtiter Dish Biofilm Formation Assay
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Cited by 2235 •
2011
The assay describes a rapid means to measure early biofilm formation in bacteria and fungi. This method uses a microtiter plate as the substratum for microbial biofilm formation, and the biofilm is visualized using crystal violet strain. The assay provides either a qualitative or quantitative assay for early biofilm formation.
03:53
Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture
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2025
This video demonstrates constructing a three-compartment cell culture system using collagen and non-reactive grease. This system is used to observe interactions between glioma cells and axons in real-time.
06:06
A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells
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Cited by 16 •
2012
Cultured muscle cells are an inadequate model to recapitulate innervated muscle in vivo. A functional motor unit can be reproduced in vitro by innervation of differentiated human primary muscle cells using rat embryo spinal cord explants. This article describes how co-cultures of spinal cord explants and muscle cells are established.
06:47
Production of Cardiac Extracellular Matrix from Adult Human Fibroblasts for Culture Dish Coating
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Cited by 1 •
2024
Fibroblasts isolated from the adult human heart were cultured to confluence on gelatin-coated dishes to produce the myocardium-specific extracellular matrix. After decellularization, this substrate can be used for the culture and study of other cardiac cells and cell-matrix interactions.
13:34
Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish
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Cited by 13 •
2019
Drug targeting to central nervous system tumors is a major challenge. Here we describe a protocol to produce an in vitro mimic of the blood-brain tumor-barrier using murine and/or human cells and discuss their relevance for the predictability of central nervous system tumor targeting in vivo.
FAQs
Agar serves as a nutrient medium that supports growth while keeping the sample distributed on a surface that can be observed. As cells multiply, they form visible colonies, making growth location easier to examine. This surface-based arrangement is particularly useful when the goal is to isolate colonies or compare biological growth under controlled conditions.
Sterility and controlled incubation establish a defined starting environment for a culture experiment. A sterile medium helps ensure that observations relate to the introduced sample, while controlled conditions make growth easier to observe consistently. Maintaining these conditions allows researchers to interpret visible colonies or cell behavior in relation to the intended experimental setup.
Streaking and spreading distribute a sample across the nutrient medium rather than leaving it concentrated in one location. This arrangement separates cells spatially as they grow, allowing distinct colonies to become visible. Researchers can then examine or isolate colonies individually, which supports microbial culture and studies requiring clearer separation of biological material.
A typical workflow places sterile nutrient medium, such as agar, into the dish before introducing the biological sample by streaking or spreading. The lidded dish is then incubated under controlled conditions so growth can occur. After incubation, researchers observe the resulting colonies or cell behavior and use those observations for the planned biological analysis.
Biologists use Petri dishes when they need a contained, observable setting for biological growth or cell behavior. Common applications include microbial culture, colony isolation, antibiotic sensitivity testing, environmental sampling, clinical microbiology, teaching, and experimental research. Their simple format supports repeated observation while keeping the sample and nutrient medium together in one accessible system.
A Petri Dish provides a nutrient-medium setting in which cultured microorganisms can be examined in an antibiotic sensitivity test. The method connects observable microbial growth with the question of how sensitive an organism is to an antibiotic. This application makes the dish useful in clinical microbiology and research where growth-based observations inform biological comparisons.