3d Culture Systems
3d Culture Systems - Related Videos
08:01
Fabrication of 3D Carbon Microelectromechanical Systems (C-MEMS)
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Cited by 6 •
2017
Long and hollow glassy carbon microfibers were fabricated based on the pyrolysis of a natural product, human hair. The two fabrication steps of carbon microelectromechanical and carbon nanoelectromechanical systems, or C-MEMS and C-NEMS, are: (i) photolithography of a carbon-rich polymer precursor and (ii) pyrolysis of the patterned polymer precursor.
01:32
Three-Dimensional Culture Systems: Spheroids and Organoids
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2026
Three-dimensional (3D) culture systems, including spheroids and organoids, support New Approach Methodology (NAM)-aligned research by providing more physiologically relevant models than conventional two-dimensional cell cultures. Because they more closely resemble the structure and cellular interactions of tissues and organs, these systems can provide physiologically relevant data before advancing to animal studies. Spheroids as Disease and Drug Screening Models Spheroids are generated when one...
10:11
Cell Co-culture Patterning Using Aqueous Two-phase Systems
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Cited by 32 •
2013
Aqueous two-phase systems were used to simultaneously pattern multiple populations of cells. This fast and easy method for cell patterning takes advantage of the phase separation of aqueous solutions of dextran and polyethylene glycol and the interfacial tension that exists between the two polymer solutions.
06:54
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
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Cited by 12 •
2012
Traditional, two dimensional cell culture techniques often result in altered characteristics with respect to differentiation markers, cytokines and growth factors. Three-dimensional cell culture in the rotating cell culture system (RCCS) reestablishes expression of many of these factors as shown here with an extravillous trophoblast cell line.
05:31
Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
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Cited by 23 •
2019
We describe protocols to prepare oocysts and purify sporozoites for studying infection of human intestinal and airway organoids by Cryptosporidium parvum. We demonstrate the procedures for microinjection of parasites into the intestinal organoid lumen and immunostaining of organoids. Finally, we describe the isolation of generated oocysts from the organoids.
FAQs
Spatial organization creates regions with different access to nutrients and oxygen, producing conditions that are not represented in conventional two-dimensional cultures. These gradients can influence how cancer cells behave throughout the model and help researchers examine tumor growth under tissue-like conditions. Consequently, experimental results may provide a more physiologically relevant view of cellular responses.
Spheroids form when cells self-assemble into three-dimensional aggregates, whereas scaffold-based models grow cells within biomaterial scaffolds or extracellular matrix. These approaches create spatial structure and cell contacts through different physical arrangements. Comparing them allows researchers to select a model suited to the tissue-like organization or microenvironmental features relevant to a particular cancer question.
Cells positioned in three dimensions can establish cell-cell contacts that differ from those in flat cultures. Those interactions contribute to spatial organization and can influence cancer-cell behavior within the model. Including these contacts helps researchers connect cellular mechanisms with tissue-level outcomes, particularly when examining tumor growth, invasion, or responses to treatment.
Two-dimensional cultures provide a simpler setting for studying cells, while 3D culture systems add tissue-like architecture, spatial organization, gradients, and cell-cell interactions. The added features can reveal behaviors that are difficult to represent on a flat surface. Using both approaches supports comparison between simplified cellular observations and more physiologically relevant cancer-model outcomes.
A model may be built from cells that self-assemble into spheroids or from cells grown within biomaterial scaffolds and extracellular matrix. These components provide the three-dimensional setting needed to organize cells and support interactions within the model. The selected arrangement determines how researchers represent tissue architecture and investigate cancer-related behavior.
Researchers can use these systems to investigate tumor growth, invasion, treatment response, and interactions with the surrounding microenvironment. Their three-dimensional organization makes it possible to examine these outcomes in a setting that more closely reflects tissue conditions than a conventional flat culture. This makes the approach useful when spatial relationships and tissue-level behavior are central to the research question.
Because these models reproduce spatial organization, nutrient and oxygen gradients, and cell-cell contacts, they can support treatment testing under more physiologically relevant conditions. Researchers can examine how cancer models respond to treatments while retaining tissue-like features that influence behavior. The resulting observations may complement findings from simpler cultures and help connect cellular responses with tissue-level outcomes.
3D culture systems can complement animal studies by linking cellular mechanisms with tissue-level outcomes before or alongside investigations in living organisms. They provide a controlled laboratory setting for examining tumor growth, invasion, microenvironmental interactions, and treatment response. Their role is therefore complementary rather than a replacement for the broader evidence obtained from animal research.