Fibroblast-derived Matrix
Fibroblast-derived Matrix - Related Videos
07:21
Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
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Cited by 3 •
2019
The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells).
02:38
Isolating Induced Neural Progenitor Cell Colonies Derived From Adult Human Fibroblasts
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2025
This video demonstrates a technique for isolating induced neural progenitor cells (iNPCs) derived from adult human fibroblasts. It outlines the steps involved in isolating the iNPC colonies and culturing them in laminin-coated multi-well plate wells in the presence of suitable culture media that facilitate the continued proliferation and expansion of the iNPC population.
09:40
A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
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Cited by 42 •
2017
The extracellular matrix plays a major role in defining the microenvironment of cells and in modulating cell behavior and phenotype. We describe a rapid method for the isolation of cell-derived extracellular matrix, which can be adapted to different scales for microscopic, biochemical, proteomic, or functional studies.
10:48
Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
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Cited by 8 •
2017
Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.
13:58
Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
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Cited by 38 •
2015
Generation of induced pluripotent stem cells provides fascinating prospects for the derivation of autologous transplants. However, progression through a pluripotent state and laborious re-differentiation still hinders clinical translation. Here we describe the derivation of adult human fibroblasts and their direct conversion into induced neural progenitor cells and the subsequent differentiation into neural lineages.
FAQs
Its organized extracellular structure presents both mechanical and biochemical cues that cancer cells can sense through contact with the surrounding scaffold. These cues influence how cells attach, spread, and move across the matrix. Using this system, investigators can examine how tissue-like organization contributes to tumor-cell behavior rather than studying cancer cells on an artificial surface alone.
Collagen and fibronectin contribute distinct structural and adhesive features to the deposited matrix. Their presence and organization help determine how cancer cells interact with the scaffold, including the extent of attachment and spreading. Examining these proteins in combination allows researchers to relate matrix composition and organization to changes in migration or invasion.
The comparison reveals how stromal remodeling may alter the tumor microenvironment. Differences between normal and cancer-associated fibroblast matrices can change the structural and biochemical cues encountered by cancer cells, helping investigators connect fibroblast state with tumor progression. This approach separates effects associated with the matrix itself from effects caused directly by the cancer cells.
Fibroblasts are first cultured so they can secrete and organize extracellular-matrix proteins, including collagen and fibronectin. The fibroblasts are then removed, leaving the deposited material as an acellular scaffold. Cancer cells can subsequently be placed on this matrix to assess responses to a fibroblast-produced environment without the continued presence of matrix-producing cells.
This model supports examination of cancer-cell adhesion, spreading, migration, and invasion. Because the cells encounter a deposited scaffold with tissue-like structural and biochemical cues, researchers can evaluate how those behaviors change with matrix source or organization. The resulting comparisons help identify stromal features that may support more aggressive interactions between cancer cells and their surroundings.
Cancer cells can be evaluated in a matrix environment that more closely reflects stromal conditions than a simplified culture surface. Investigators can compare treatment responses across matrices produced by different fibroblast populations or with different organization. Such experiments help determine whether extracellular-matrix context influences observed responses and whether stromal remodeling should be considered when interpreting treatment outcomes.