Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
 

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Article DOI: 10.3791/60304-v
December 26th, 2019

Chapters

Summary December 26th, 2019

The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells).

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter