Acute Muscle Injury
Acute Muscle Injury - Related Videos
09:09
Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
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Cited by 141 •
2013
We describe models of moderate and severe ischemia-reperfusion-induced kidney injury in which the mice undergo unilateral renal pedicle clamping followed by simultaneous or delayed contralateral nephrectomy, respectively. These models consistently give rise to renal dysfunction and post-injury fibrosis, but injury severity and survival are dependent on mouse background, age and surgical equipment.
07:55
Pressure Controlled Ventilation to Induce Acute Lung Injury in Mice
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Cited by 7 •
2011
A murine model for ventilator induced lung injury is an important tool to study an acute lung injury in vivo. Here, we report an easy applicable in situ model for acute lung injury using high-pressure mechanical ventilation to induce acute failure of the lung.
07:13
Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
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Cited by 14 •
2013
The method presented here comprises the precise injury of live zebrafish embryos with high-energy laser pulses and the subsequent analysis of these injuries and their recovery with time. We also show how genetically labeled single or groups of skeletal muscle cells can be tracked during and after laser light induced damage.
10:45
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
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Cited by 27 •
2017
This protocol describes histological analyses of the livers from zebrafish larvae that have been treated with 2% ethanol for 24 h. Such acute ethanol treatment results in hepatic steatosis and swelling of the hepatic vasculature.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
FAQs
Satellite cells respond to the injured environment by moving through a defined regenerative sequence: activation, proliferation, differentiation, and fusion with existing muscle fibers. This progression allows researchers to examine how a relatively quiescent muscle cell population becomes involved in rebuilding damaged tissue and how cellular behavior supports restoration of muscle structure.
Damaged muscle fibers and nearby tissues generate local signals that initiate inflammation. This response provides an early tissue context for subsequent repair, including satellite-cell activation. Studying these signals helps reveal how muscle coordinates damage recognition with regenerative activity rather than treating inflammation and regeneration as completely separate processes.
Muscle repair provides a post-injury model that can be compared with embryonic muscle formation. Both settings allow investigators to examine how cells, extracellular matrix, and signaling pathways control muscle growth and remodeling. The comparison can identify shared regulatory principles while also highlighting how mature tissue responds differently from developing embryonic tissue.
The extracellular matrix and signaling pathways help organize how muscle tissue changes during repair and remodeling. In developmental biology, examining these components alongside satellite-cell behavior shows how local tissue structure and molecular communication influence cellular decisions. This perspective helps connect individual cell responses with broader changes in muscle organization after injury.
Researchers use the injury response to follow linked events from damaged fibers and local signaling through inflammation and satellite-cell activity. They can then compare these repair-associated changes with developmental muscle formation. This model is valuable because it connects cellular behavior, tissue remodeling, and regenerative outcomes within a single biological context.
Findings from this model can inform investigations of congenital muscle disorders, regenerative medicine, and approaches intended to improve recovery after injury. Its developmental-biology relevance comes from revealing how muscle cells and their surrounding environment regulate growth and repair. These insights may guide research into strategies that support more effective tissue regeneration.