Optic Cup Removal
Optic Cup Removal - Related Videos
07:26
4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis
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Cited by 1 •
2021
This protocol describes an approach for in toto labeling and multidimensional imaging of zebrafish early eye development. We describe labeling, embedding, and four dimensional (4D) imaging using laser scanning confocal microscopy, and considerations for optimizing acquisition of datasets for dissecting mechanisms of optic cup morphogenesis.
02:45
Retinal Cup Extraction from Mouse: A Surgical Procedure to Obtain Intact Retinal Cup from Mouse Eye
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2025
This video describes a protocol to isolate the retinal cup without the outer covering layers from the eyeball of a mouse. The extracted retinal tissue can help study various metabolic pathways occurring in the retinal cells.
11:58
Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers
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Cited by 10 •
2013
We discussed the synthesis of individual graphitic nanocups using a series of techniques including chemical vapor deposition, acid oxidation and probe-tip sonication. By citrate reduction of HAuCl4, the graphitic nanocups were effectively corked with gold nanoparticles due to the chemically reactive edges of the cups.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
FAQs
The optic cup normally exchanges inductive signals with the surface ectoderm and surrounding tissues. Its removal interrupts this communication, allowing researchers to examine which developmental events depend on the cup and which proceed independently. Comparing altered development after removal with normal eye formation helps identify tissue interactions that guide ocular patterning and differentiation.
This manipulation can separate contributions to neural retina formation, retinal pigment epithelium development, lens formation, and the development of other eye tissues. Because the optic cup is removed during an early embryonic stage, researchers can assess whether each tissue requires direct interaction with the cup or responds to signals from other embryonic tissues.
Removal at an early embryonic stage tests tissue relationships before ocular structures complete their normal development. At this point, the optic cup, surface ectoderm, and surrounding tissues are engaged in reciprocal signaling. Altering the interaction early therefore helps reveal developmental dependencies that may be obscured after retinal, lens, and related tissues have already differentiated.
The procedure begins with an early embryonic eye and uses microsurgical manipulation to excise the optic cup. Researchers then examine how development proceeds without the cup and compare the resulting ocular tissues with normal development. The essential experimental contrast is the presence or absence of optic-cup interactions during subsequent tissue formation.
Changes in the neural retina, retinal pigment epithelium, lens, or other eye tissues indicate that their development is influenced by signals from the optic cup or by interactions that the cup normally supports. Interpreting these outcomes requires distinguishing a missing tissue contribution from disrupted communication among the remaining embryonic tissues.
In developmental biology, this technique provides a direct test of how tissue interactions control organ patterning and differentiation. It also contributes to research on regeneration by showing how developmental signals influence the formation or restoration of ocular tissues. The resulting evidence helps connect embryonic tissue relationships with broader principles of eye development.