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JoVE Journal
Neuroscience

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4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis
 

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

Article DOI: 10.3791/62155-v
May 26th, 2021

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Summary May 26th, 2021

This protocol describes an approach for in toto labeling and multidimensional imaging of zebrafish early eye development. We describe labeling, embedding, and four dimensional (4D) imaging using laser scanning confocal microscopy, and considerations for optimizing acquisition of datasets for dissecting mechanisms of optic cup morphogenesis.

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