Kernel Screening Assay

A Kernel Screening Assay is a targeted laboratory method for examining individual cereal or plant kernels to identify measurable differences in quality, viability, stress response, or contamination, making it useful for environmental and agricultural assessment. The assay evaluates kernels under defined analytical or biological conditions and compares each sample’s signal with controls to classify affected and unaffected specimens. In environmental research, this screening supports rapid evaluation of exposure effects, contaminant presence, and variation across samples, helping researchers prioritize specimens for confirmatory chemical analysis, ecological studies, or crop-management decisions.

Kernel Screening Assay - Related Videos

Research

JoVE Journal - Immunology and Infection

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

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Cited by 39 •

2012

The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.

Small Molecule Nematicides Screening Assay: A Medium Throughput Method to Screen Potential Nematicides Against Ditylenchus dipsaci

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2025

In this video, we demonstrate a screening assay to identify the efficacy of small-molecule nematicides against nematodes. Nematicides are considered effective if they cause a decrease in the number of mobile nematodes in the sample after exposure.

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

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Cited by 45 •

2014

We present a rapid and inexpensive screening method for identifying transcriptional regulators using high-throughput robotic transfections and a homemade dual-glow luciferase assay. This protocol rapidly generates direct side-by-side functional data for thousands of genes and is easily modifiable to target any gene of interest.

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

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Cited by 1 •

2017

Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

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Cited by 7 •

2012

A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.

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