Cell Panning
Cell Panning - Related Videos
13:45
Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
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Cited by 84 •
2011
The Virochip is a pan-viral microarray designed to simultaneously detect all known viruses as well as novel viruses on the basis of conserved sequence homology. Here we demonstrate how to run a Virochip assay to analyze clinical samples for the presence of both known and unknown viruses.
06:25
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
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Cited by 25 •
2019
This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.
07:47
Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
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2023
Here, a protocol is presented in which multiple bioinformatic tools are combined to study the biological functions of TMEM200A in cancer. In addition, we also experimentally validate the bioinformatics predictions.
10:25
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
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Cited by 7 •
2019
Here, we present a protocol for immunophenotypic characterization and cytokine induced differentiation of cord blood derived CD34+ hematopoietic stem and progenitor cells to the four myeloid lineages. The applications of this protocol include investigations on the effect of myeloid disease mutations or small molecules on myeloid differentiation of the CD34+ cells.
15:00
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
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Cited by 44 •
2016
Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis.
FAQs
Selectivity comes from differential retention: cells or molecules that recognize the immobilized target remain after incubation and washing, whereas nonbinding material is removed. This links the final population to target-specific attachment rather than simple recovery from the starting sample. In infection studies, that distinction helps reveal reagents or immune-cell populations directed toward infected-cell surfaces or disease-associated markers.
The target determines which recognition properties can be enriched. Using pathogen-infected cells or a disease-associated surface marker focuses selection on features displayed in that biological state, while target cells provide a cellular context for recognition. Consequently, the recovered population can support investigation of host-pathogen interactions and cellular recognition within a biologically relevant setting.
Cell panning can begin with either a cell suspension or a phage-displayed library, but the selected output differs in form. A cell suspension yields enriched cells for population analysis, whereas a library can yield enriched binding clones, such as antibody or ligand candidates. This flexibility allows the same selection principle to address cellular composition and reagent discovery.
At a basic level, the workflow proceeds through target immobilization, addition of the suspension or library, incubation, washing, and recovery. Each stage has a distinct purpose: immobilization presents the recognition target, incubation permits attachment, washing removes unbound material, and recovery preserves specifically bound cells or clones for analysis or enrichment. The sequence connects experimental input to selected output.
An experiment requires an immobilized target, a cell suspension or phage-displayed library, and a way to separate unbound from bound material through washing. The target may be a cell population or a surface marker relevant to the question. These components should match whether the goal is enriching immune cells, identifying antibodies, or finding ligands.
In immunology, recovered cells can help identify immune-cell populations associated with particular recognition properties. In infection research, selections against pathogen-infected cells can identify antibodies or ligands that bind disease-associated surfaces. The resulting enriched material supports studies of cellular recognition and host-pathogen interactions, while also providing candidates for diagnostic or targeted therapeutic development.
Enrichment indicates recovery of material capable of attaching to the selected target, but it does not by itself establish the full biological function of that material. Further analysis is needed to characterize recovered cells or clones. This distinction matters when interpreting panning results for diagnostics or therapeutic development, where target binding is an initial selection criterion.