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JoVE Journal
Immunology and Infection

This content is Open Access.

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
 

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

Article doi: 10.3791/59709
July 10th, 2019

Summary July 10th, 2019

This real-time RT-PCR using dsDNA intercalating dye is suitable to diagnose lyssavirus infections. The method begins with RNA extracted from rabies suspected ante-mortem or post-mortem samples, detailing master mix preparation, RNA addition, setup of the real-time machine and correct interpretation of results.

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