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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

DOI:
10.3791/53911-v

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15:00 min

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June 14, 2016

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, ,
1Department of Chemistry and Biochemistry,University of Oklahoma

Chapters

  • 00:05Title
  • 01:23Mouse Tissue Section Preparation
  • 02:31Cell Culture
  • 04:25Single-probe Fabrication
  • 06:18Build the Integrated Single-probe MS Setup
  • 07:41Ambient Mass Spectrometry Imaging (MSI)
  • 10:02In-situ Live SCMS
  • 11:31Results: The Single-probe System Was Successfully Established for Mass Spectrometry Analysis of Live Single Cells and Mass Spectrometry Imaging of Biological Tissues
  • 12:47Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis.

Tags

Keywords: Single-probeMass Spectrometry ImagingSingle Cell AnalysisAmbient ConditionsLive Single CellsHigh Spatial ResolutionMultifunctional DeviceMicroscale SamplingSpar AnalysisLive MicrocoloniesBiofilmsMouse OrganCryo-microtomeHeLa CellsCell CultureCell PassagingTrypsin

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