Epithelial Damage
Epithelial Damage - Related Videos
11:49
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
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Cited by 5 •
2014
Human infection by Entamoeba histolytica leads to amoebiasis, a major cause of diarrhea in tropical countries. Infection is initiated by pathogen interactions with intestinal epithelial cells, provoking the opening of cell-cell contacts and consequently diarrhea, sometimes followed by liver infection. This article provides a model to assess the early host-pathogen interactions to improve our understanding of amoebiasis pathogenesis.
08:51
Mammary Epithelial Transplant Procedure
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Cited by 23 •
2010
This article demonstrates the procedure developed by DeOme KB et al. (1959) and the sparing procedure developed by Brill B et al. (2008) for clearing the 4th inguinal mammary fat pad of a pubescent mouse in preparation for transplantation of mammary fragments, mammary epithelial cells, or mammary tumor cells.
10:09
Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
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Cited by 10 •
2013
Tissue-specific analysis of a hair follicle regeneration model using lentivirus to mediate gain- or loss-of-function.
03:50
Hematoxylin and Eosin Staining to Assess Colon Damage in DSS-Induced Colitis
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2025
This video demonstrates the hematoxylin and eosin (H&E) staining method to evaluate colon damage in a murine model of DSS-induced colitis — an inflammatory bowel disease. Upon isolating the colon from a DSS-treated mouse and obtaining sections of the tissue, H&E staining of the sections helps identify the inflammation-mediated tissue damage.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
FAQs
Cell-cell junctions help maintain the integrity of epithelial layers. When injury weakens these connections, tissue permeability can increase, allowing greater movement across the barrier and exposing molecules beneath the epithelial surface. In infection research, this change is important because altered permeability may make it easier to study how barrier disruption contributes to pathogen passage and inflammatory signaling.
Exposed molecules beneath an injured epithelial layer can activate innate immune receptors. This recognition initiates signaling that promotes cytokine release and recruitment of immune cells to the affected tissue. The mechanism links a physical barrier defect to an immune response, helping explain how local epithelial injury can produce broader inflammation during host responses to infection.
The immune activation associated with epithelial injury can help the host respond to threats at barrier surfaces by promoting cytokine signaling and immune-cell recruitment. However, excessive inflammation may intensify tissue injury rather than resolve it. This opposing relationship makes epithelial damage relevant to research on infectious disease progression, where protection and inflammation must be considered together.
Studying epithelial damage can reveal how pathogens cross skin or mucosal barriers, how host defenses respond after barrier disruption, and how inflammatory signals develop in affected tissues. These observations help connect epithelial integrity with infection progression and immune activity, providing a framework for investigating why barrier failure may influence disease outcomes.
Airway and intestinal epithelia are barrier surfaces where tissue integrity influences interactions between the host and infectious threats. Research on their damage can clarify how permeability changes, innate receptor activation, cytokine release, and immune-cell recruitment shape mucosal responses. This context supports efforts to understand protective immunity while limiting inflammation that could further compromise the barrier.
Barrier injury creates a research context for examining how damaged epithelial tissues recover and regain protective function. Wound-repair studies can therefore connect the initial loss of integrity with strategies intended to preserve or restore barrier function. In immunology and infection, this is significant because maintaining the barrier may help reduce pathogen access and limit inflammation-associated tissue injury.