Intestinal Ligated Loop
Intestinal Ligated Loop - Related Videos
05:59
Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
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Cited by 15 •
2011
M cells in a specialized follicle-associated epithelium covering Peyer’s patches play an important role for the mucosal immunosurveillance in gut-associated lymphoid tissue. Here we described the evaluation method for bacterial transcytosis by M cells in vivo. This method provides a method to understand M-cell function in the immune system.
17:15
Catheterization of Intestinal Loops in Ruminants
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Cited by 4 •
2009
We describe a novel surgical method for catheterizing 'intestinal loops' within the ileum of sheep. Once animals have recovered from surgery and have cleared antibiotics and analgesics, multiple treatments can be deposited directly in loops via the catheters.
04:58
Mouse Ileal Loop Model: A Surgical Model to Study Intestinal Permeability in the Mouse
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2025
This video demonstrates the generation of an ileal loop model to study intestinal permeability in a mouse model. This method provides a live model to understand the epithelial barrier function and consequent immune response.
04:00
Generation of Mouse Proximal Colon Loop Model: A Surgical Procedure to Generate Proximal Colon Loop and Measure Intestinal Permeability by Detection of Fluorescent Markers in Serum Following Intraluminal Injection
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2025
In this video, we describe a surgical procedure to generate mouse proximal colon loop or pcLoop segment followed by intraluminal injection of fluorescent markers into the segment to study the intestinal epithelial permeability.
09:21
A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
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Cited by 4 •
2018
Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.
FAQs
Isolation confines a test substance, microorganism, or formulation to a selected region, making it easier to connect local exposure with specific physiological or pathological changes. Investigators can then assess fluid movement, absorption, permeability, or tissue response within that segment. This controlled localization helps distinguish effects associated with the treatment from broader intestinal responses.
Ligatures create the enclosed intestinal loop that retains the introduced material within a defined section. This arrangement supports localized interaction between the intestinal tissue and the test substance, microorganism, or formulation. Measurements of luminal changes and tissue responses can therefore be interpreted in relation to a controlled exposure site, which is valuable for transport and host-pathogen studies.
The model can reveal changes in fluid movement, absorption, permeability, and tissue response after localized intestinal exposure. These outcomes provide complementary information: luminal changes reflect movement within the enclosed segment, while tissue responses indicate how the intestinal wall reacts. Together, they help characterize physiological effects, pathological responses, and interactions relevant to gastrointestinal research.
Surgeons first select the intestinal region to be examined and place ligatures around the chosen sections to create an enclosed loop. They then introduce the test substance, microorganism, or formulation into that isolated segment. Subsequent assessment focuses on measurable luminal or tissue changes, allowing the exposure to be linked with localized intestinal outcomes.
Researchers can apply the intestinal ligated loop approach when they need to examine how a drug or vaccine formulation interacts with a localized intestinal region. The enclosed segment permits assessment of absorption, permeability, and tissue response after exposure. Findings can support gastrointestinal delivery studies and contribute evidence during therapeutic development.
In medicine and pharmacology, the technique connects a controlled intestinal exposure with measurable physiological or pathological outcomes. It can be used to investigate intestinal transport, delivery of drugs or vaccines, inflammation, and host-pathogen interactions. By examining localized responses, researchers obtain evidence that can inform gastrointestinal research and the development of therapeutic formulations.