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Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
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Chapters
- 00:05Title
- 01:12Preparation of Bacterial Cells
- 02:26Ligated Peyer’s Patch Loop Assay
- 03:42Whole Mount Staining and Confocal Microscopic Analysis of Peyer’s Patch
- 05:12Localization of Fluorescent S. Typhimurium and GP2 in M Cells
- 05:36Conclusion
M cells in a specialized follicle-associated epithelium covering Peyer’s patches play an important role for the mucosal immunosurveillance in gut-associated lymphoid tissue. Here we described the evaluation method for bacterial transcytosis by M cells in vivo. This method provides a method to understand M-cell function in the immune system.
