Msc Hydrogels
Msc Hydrogels - Related Videos
09:19
Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model
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Cited by 1 •
2020
Stem cell-based therapy has emerged as an efficient strategy to repair injured cardiac tissues after myocardial infarction. We provide an optimal in vivo application for stem cell transplantation using gelatin hydrogels that are able to be enzymatically cross-linked.
11:13
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
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Cited by 47 •
2017
Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
08:33
Hydrogel Synthesis
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2023
Source: Amber N. Barron, Ashlea Patterson, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Hydrogels are a versatile class of cross-linked polymers produced through relatively simple procedures and with generally inexpensive materials. They can be formed from solution and involve a polymer backbone formed from monomer reagents, an initiator which makes the polymer reactive and a crosslinking species which binds the polymer chains...
11:05
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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Cited by 21 •
2017
The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
FAQs
Crosslinking determines how tightly the polymer network is connected, which affects stiffness, porosity, degradation, cell retention, and movement of nutrients or signaling molecules. Adjusting these properties helps researchers create an environment suited to a particular biomedical purpose. The resulting matrix can therefore influence how well MSCs survive and how they respond within the engineered tissue environment.
These properties control both the physical support available to MSCs and the substances that can reach or leave the hydrogel. Stiffness changes the mechanical environment, porosity influences transport, and degradation alters how long the network persists. Together, they affect cell retention, survival, exposure to signals, and the conditions under which differentiation is studied.
MSC differentiation can be influenced by the engineered environment surrounding the cells. In a hydrogel, matrix stiffness, pore structure, degradation, and diffusion of signaling molecules collectively shape cellular conditions. Researchers can tune these features to examine how MSCs respond to tissue-like settings or to support desired regenerative outcomes, although the specific response depends on the selected hydrogel design.
Injectable and implantable platforms differ mainly in how they are introduced and positioned for treatment or study. An injectable formulation is investigated for localized delivery, whereas an implantable construct is placed as a defined material or tissue-supporting platform. Both approaches can combine MSC activity with a tunable matrix, but their use depends on the intended biomedical application.
Development begins by matching the hydrogel’s polymer network properties to the intended role of the MSCs. Researchers consider stiffness, porosity, degradation, cell retention, and diffusion of nutrients or signaling molecules, then select an injectable or implantable format when appropriate. These decisions determine whether the platform is better suited to tissue repair, cell-based therapy, drug delivery, or modeling.
MSC hydrogels are being investigated for tissue repair, regenerative medicine, drug delivery, and cell-based therapies. They may provide localized treatment by keeping cells or therapeutic signals within an engineered environment. In research, the same platforms can model how MSCs respond to tissue-like conditions, helping connect biomaterial design with cellular behavior and potential treatment outcomes.