Colonic Distension
Colonic Distension - Related Videos
03:20
Electrophysiological Recording from Colonic Afferent Nerves in an Ex Vivo Rat Colon
0 Views •
2025
This video demonstrates a method to record the activity of colonic afferent nerves in an ex-vivo rat colon, cannulated at both ends and infused with saline to increase intraluminal pressure. As pressure increases, the nerve signal rises, indicating its stimulation.
04:00
Generation of Mouse Proximal Colon Loop Model: A Surgical Procedure to Generate Proximal Colon Loop and Measure Intestinal Permeability by Detection of Fluorescent Markers in Serum Following Intraluminal Injection
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2025
In this video, we describe a surgical procedure to generate mouse proximal colon loop or pcLoop segment followed by intraluminal injection of fluorescent markers into the segment to study the intestinal epithelial permeability.
10:32
In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
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Cited by 8 •
2016
Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
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Cited by 64 •
2016
The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.
07:53
Quantification of Colonic Stem Cell Mutations
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Cited by 1 •
2015
We report significant improvements for the reproducible measurement of somatic colonic stem cell mutations after exposure of mice to potential DNA damaging agents.
FAQs
Stretch-sensitive receptors in the colonic wall can transmit signals through enteric, spinal, and vagal afferent pathways. These routes connect local gastrointestinal control with central neural processing, allowing mechanical changes in the colon to influence motility, secretion, autonomic activity, and visceral sensation. Studying the pathways together helps researchers examine gut-brain communication at multiple levels.
When the colonic wall is stretched, its receptors provide a mechanical input that can initiate reflex responses. Those responses may alter intestinal motility and secretion while also engaging autonomic activity. Consequently, the same distension-related signal can contribute both to local regulation of gastrointestinal function and to neural processing associated with visceral sensation.
Spinal and vagal afferent pathways provide distinct routes through which colonic mechanical signals participate in gut-brain communication. Their involvement allows researchers to investigate how intestinal conditions influence central neural processing, autonomic responses, and perception. Considering these pathways alongside enteric circuits gives a broader view than examining colonic motility or sensation in isolation.
Colonic distension is useful for examining why mechanical intestinal signals may be associated with different levels of visceral sensation or pain. In studies of visceral hypersensitivity, the response to distension helps investigate disease-related changes in pain perception and neural processing. This makes the approach relevant to functional bowel disorders and the mechanisms underlying their symptoms.
Controlled colonic distension provides a structured way to examine responses to a defined mechanical change in the colon. Researchers can assess resulting effects on motility, secretion, autonomic activity, or pain perception, depending on the study objective. The approach supports direct investigation of how gastrointestinal mechanical signals are converted into physiological and sensory outcomes.
Studies can evaluate several outcome domains rather than relying on a single readout. These include changes in gastrointestinal motility and secretion, autonomic activity, visceral sensation, and pain perception. Comparing these responses helps determine whether a mechanical signal primarily affects local gut function, neural regulation, sensory processing, or several of these systems simultaneously.
The colon provides mechanical information that can engage enteric, spinal, and vagal neural pathways, linking gastrointestinal conditions with nervous-system activity. Investigating this signaling helps explain how intestinal events may influence behavior, homeostasis, and disease-related pain. In neuroscience, the method therefore connects peripheral gut mechanics with broader questions about sensory processing and autonomic regulation.