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Quantification of Colonic Stem Cell Mutations
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Developmental Biology
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JoVE Journal Developmental Biology
Quantification of Colonic Stem Cell Mutations

Quantification of Colonic Stem Cell Mutations

DOI:
10.3791/53240-v

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07:53 min

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September 25, 2015

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1Department of Pharmaceutical Sciences,University of Pittsburgh

Chapters

  • 00:05Title
  • 02:10Preparation of Glucose-6-phosphate Dehydrogenase (G6PD) Staining Mixture
  • 03:56Tissue Section Staining
  • 05:19Determination of Mutation Frequency
  • 06:14Results: Colonic Stem Cell Mutation Frequency as Determined by Analysis of G6PD Mutant Crypts
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

We report significant improvements for the reproducible measurement of somatic colonic stem cell mutations after exposure of mice to potential DNA damaging agents.

Tags

Keywords: Stem Cell MutationsGlucose-6-phosphate Dehydrogenase GeneG6PD EnzymeOptimal Cutting Temperature Compound (OCT)CryptMutation FrequencyEnzymatic Assay

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