E-plate Technology
E-plate Technology - Related Videos
13:29
Jet Impinging on an Inclined Plate
0 Views •
2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The goal of this experiment is to demonstrate how a fluid flow exerts forces on structures by conversion of dynamic pressure into static pressure. To this end, we will make a plane jet impinge on a flat plate and will measure the resulting pressure distribution along the plate. The resultant force will be estimated by integrating the product between the...
18:00
Aseptic Laboratory Techniques: Plating Methods
0 Views •
Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
01:26
Technological Change
0 Views •
2024
Total Factor Productivity (TFP) measures the efficiency with which inputs are transformed into outputs in production. It is the essence of economic growth, driven by technological advancement. Consider the agricultural sector, where production requires vast amounts of human labor and work animals. Today, modern farm machinery and agriculture technologies have revolutionized how we cultivate crops and produce food much more efficiently. TP = A*f(K, L) TFP is represented as a multiplier 'A' in...
01:31
Hybridoma Technology
0 Views •
2023
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy. Hybridoma Selection Commonly used fusion techniques — electroporation, polyethylene glycol...
07:06
Principles of Site-Specific Recombinase (SSR) Technology
0 Views •
Cited by 14 •
2008
The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular biology, and has proven itself as a valuable tool for assessing gene function in transgenic animals. This interview discusses the mechanism of site specific recombination by Cyclization recombinase (Cre) and how the use of this enzyme has led to the development of conditional mutagenesis, which has significant advantages over traditional knock out strategies.
FAQs
Microelectrodes detect changes in electrical impedance at the cell-covered surface. As neural cells attach, spread, proliferate, differentiate, or alter their morphology, their interaction with the electrode interface changes, producing a corresponding electrical signal. Recording these changes continuously creates a quantitative time course that captures cellular dynamics rather than relying on a single endpoint measurement.
Label-free monitoring avoids fluorescent or radioactive labels that could otherwise be part of the measurement design. Because the cells can be followed continuously through electronic sensing, researchers can observe changes across time while examining viability, growth, differentiation, or treatment responses. This kinetic perspective is valuable when neural behavior develops gradually or changes during an experiment.
Several behaviors contribute to the measured signal, including cell attachment, spreading, proliferation, differentiation, and changes in morphology. These processes modify how cells occupy and interact with the microelectrode-containing surface, so the impedance trace can reflect evolving cell state. In neuroscience experiments, the resulting pattern supports quantitative assessment of neural cell growth and response dynamics.
A typical workflow places neuronal or neural stem cells in disposable plates containing microelectrodes and records electronic measurements as the cells develop. Researchers can then follow the signal over time while assessing viability, growth, differentiation, or morphological responses. Experimental conditions may include drug exposure or environmental changes, allowing cellular effects to be evaluated as continuous quantitative measurements.
This approach is useful when investigators need kinetic information about neural cell behavior rather than only a final observation. It can support studies of neuronal or neural stem cell responses to drugs and environmental conditions, including changes related to viability, growth, or differentiation. The method therefore helps evaluate neuroactive compounds and characterize cellular responses under controlled experimental conditions.
Continuous impedance measurements can provide quantitative evidence of how neural cells behave within disease models or during evaluation of cell-based therapies. By tracking changes associated with growth, differentiation, viability, or morphology, researchers can compare cellular responses across experimental conditions. These time-resolved observations may strengthen assessment of treatment effects and the behavior of therapeutic neural cell populations.