Whole-cell Patch
Whole-cell Patch - Related Videos
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Cited by 1 •
2017
We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.
13:07
One-channel Cell-attached Patch-clamp Recording
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Cited by 17 •
2014
Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes.
03:12
Establishing a Whole-Cell Configuration through the Patch Clamp Method
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2025
This video demonstrates the whole-cell patch clamp technique in a mouse brain slice. This technique establishes a stable connection between micropipette and neuron membrane, which is further disrupted to establish a whole-cell configuration to study the neuron's health and physiological responses.
10:36
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
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Cited by 6 •
2012
We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.
09:49
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
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Cited by 29 •
2013
There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.
FAQs
A tight seal is essential because it electrically isolates the recording site from surrounding solution, allowing measured currents or voltage changes to reflect activity in the selected cell. The subsequent gentle suction step ruptures the membrane directly beneath the pipette, creating intracellular access. This combination makes single-cell measurements sufficiently controlled for studying neuronal signaling mechanisms.
Whole-cell patch recordings can operate in two complementary control modes. In voltage control, the experimenter sets membrane voltage and measures the ionic currents that result. In current control, injected current is controlled while resulting voltage changes, including action potentials, are observed. Choosing between these modes helps separate channel behavior from overall neuronal excitability.
Once the membrane beneath the pipette is opened, researchers can control electrical conditions at the cell and monitor responses that depend on its ion channels and synaptic inputs. This intracellular access links measured electrical signals to the mechanisms shaping excitability, allowing investigators to examine how cellular properties contribute to neuronal communication.
Researchers position a glass micropipette against a selected cell, establish a tight seal with the membrane, and apply gentle suction to rupture the membrane beneath the pipette. They then use voltage or current control while recording ionic currents, action potentials, or synaptic responses. This workflow progresses from cell contact to intracellular electrical measurement.
Measured ionic currents can reveal how ion channels contribute to a cell's electrical behavior, whereas action potentials report neuronal excitability. Synaptic responses provide information about inputs received by the neuron. Considering these readouts together helps researchers connect membrane mechanisms with signaling and communication at the single-cell level.
It is useful when investigators need cellular detail for questions involving neural circuits, pharmacological effects, brain function, or neurological disease. Whole-cell Patch recordings from individual cells allow researchers to evaluate how electrical properties, ion channels, and synaptic inputs relate to those broader questions, connecting cellular mechanisms with neuronal signaling and communication.