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Journal / Biology / One-channel Cell-attached Patch-clamp Recording
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One-channel Cell-attached Patch-clamp Recording

Article DOI: 10.3791/51629 • 13:07 min • June 9th, 2014
June 9th, 2014 •
1, 2, 1, 3, 4
1Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY, 2Department of Biochemistry, SMBS, University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department, The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY

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Summary
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Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes.

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One-channel Cell-attached Patch-clamp Recording Ion Channel Proteins Ionic Flux Operational Dynamics NMDA Receptor HEK293 Cells Cortical Neurons Ligand-gated Ion Channel Conductance Properties Open-closed Conformations Activation Mechanism Health And Disease
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