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1Graduate Program in Neuroscience, SMBS,University at Buffalo, SUNY, 2Department of Biochemistry, SMBS,University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department,The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS,University at Buffalo, SUNY
Chapters
- 00:05Title
- 01:53Cell Culture and Protein Expression
- 03:06Cell-Attached Patch-Clamp Recording
- 07:21Data Preprocessing and Idealization
- 11:22Results: One-Channel Recordings
- 12:25Conclusion
Described here is a procedure for obtaining long stretches of current recording from one ion channel with the cell-attached patch-clamp technique. This method allows for observing, in real time, the pattern of open-close channel conformations that underlie the biological signal. These data inform about channel properties in undisturbed biological membranes.
