J Per K
J Per K - Related Videos
09:42
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
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Cited by 17 •
2016
Channels for the transportation of water molecules in enzymes influence active site solvation and catalysis. Herein we present a protocol for the engineering of these additional catalytic motifs based on in silico computer modeling and experiments. This will enhance our understanding of the influence of solvent dynamics on enzyme catalysis.
05:54
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
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Cited by 31 •
2013
A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.
13:56
Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
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Cited by 4 •
2012
Cataract is the leading cause of blindness in the world. Solar ultraviolet radiation (UVR) is the main risk factor for cataract development. An animal model of far UVR-B induced cataract was developed. In this article we describe methods for investigation of cataract formation: exposure to UVR, quantitative RT-PCR and immunohistochemistry.
10:04
Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
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Cited by 27 •
2017
Amyloid deposition is a hallmark of different diseases and afflicts many different organs. This paper describes the application of luminescent conjugated oligothiophene fluorescence staining in combination with fluorescence microscopy techniques. This staining method represents a powerful tool for detection and exploration of protein aggregates in both clinical and scientific setups.
09:17
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
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Cited by 2 •
2018
Here we describe a co-immunoprecipitation protocol to study protein-protein interactions between endogenous nuclear proteins under hypoxic conditions. This method is suitable for demonstration of the interactions between transcription factors and transcriptional co-regulators at hypoxia.
FAQs
The relation dS = δQrev/T expresses how much entropy change accompanies a given amount of reversible heat transfer at temperature T. The same heat transfer therefore contributes differently depending on the temperature at which it occurs. Using J/K lets thermodynamic analyses quantify entropy changes and follow how energy becomes dispersed within a system.
Their shared units arise because both relate energy to temperature, but their meanings differ. Heat capacity describes how much energy is associated with a temperature change through C = δQ/dT. Entropy change instead follows reversible heat transfer through dS = δQrev/T. Consequently, J/K can describe either thermal energy storage or thermodynamic energy dispersal.
Statistical mechanics supplies a microscopic context for macroscopic entropy. It connects the measured thermodynamic quantity to the system's microscopic states, while thermodynamics describes the observable energy and temperature relationships. This connection helps physicists interpret entropy measurements as more than numerical values, linking large-scale thermal behavior with underlying microscopic possibilities.
In calorimetry, quantities expressed in J/K help characterize how a material stores thermal energy as its temperature changes. The heat-capacity relation C = δQ/dT provides the relevant connection between transferred energy and temperature variation. Such measurements allow physicists to compare thermal behavior among materials and analyze thermal systems quantitatively.
The expression dS = δQrev/T specifically uses reversible heat transfer, so the reversible condition is part of the entropy calculation rather than an optional detail. Keeping that condition explicit prevents the relation from being applied without regard to the process described. This distinction supports consistent thermodynamic analyses of materials, phase changes, and thermal systems.
J/K measurements provide a common scale for tracking entropy and heat-capacity behavior in systems undergoing significant thermal changes. In studies of phase changes, they help characterize how the system stores or disperses energy. In engine analysis, the same units support thermodynamic accounting of heat-related changes and energy dispersal across the thermal system.
The unit links several levels of thermal analysis. At the experimental level, calorimetry measures how materials store energy. At the thermodynamic level, entropy calculations track energy dispersal during heat transfer. At the statistical-mechanical level, entropy connects those observations with microscopic states. Together, these uses make J/K relevant to materials, phase changes, engines, and thermal systems.