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Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

DOI:
10.3791/53168-v

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09:42 min

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January 16, 2016

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1Science for Life Laboratory, School of Biotechnology,KTH Royal Institute of Technology

Chapters

  • 00:05Title
  • 01:00In Silico Computer Modeling
  • 02:02Model of Active Site Solvation and Water Access
  • 03:02In Silico Enzyme Engineering to Modify Water Patterns and Water Dynamics
  • 03:56Membrane Extracton Using a Normal Centrifuge and Protein Purification
  • 05:10Kinetics
  • 06:20Extraction and Thermodynamic Analysis
  • 08:20Results: Impact of Water Dynamics on Enzyme Catalysis
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

Channels for the transportation of water molecules in enzymes influence active site solvation and catalysis. Herein we present a protocol for the engineering of these additional catalytic motifs based on in silico computer modeling and experiments. This will enhance our understanding of the influence of solvent dynamics on enzyme catalysis.

Tags

Keywords: Enzyme CatalysisSolvent DynamicsMembrane EnzymesProtein StructureMolecular DynamicsWater TransportCAVER 3.0Tunnel EngineeringBiocatalysis

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