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Kwantitatieve analyse van Autophagy met Advanced 3D Fluorescentiemicroscopie

Article DOI: 10.3791/50047-v • 09:59 min • May 3rd, 2013
May 3rd, 2013 •
1,2, 2, 2,3, 4,5, 5,6, 1,2,5
1Department of Biochemistry and Molecular Medicine, University of California, Davis, 2NSF Center for Biophotonics Science & Technology, University of California, Davis, 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis, 5UC Davis Comprehensive Cancer Center, University of California, Davis, 6Department of Biological Chemistry, University of California, Davis

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Autofagie is een alomtegenwoordige proces waarmee cellen om eiwitten en organellen degraderen en te recyclen. We passen geavanceerde fluorescentie microscopie te visualiseren en kwantificeren van de kleine, maar essentiële, fysieke veranderingen geassocieerd met de inductie van autofagie, waaronder de vorming en verspreiding van autofagosomen en lysosomen, en hun fusie in autolysosomes.

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Cellular Biology Biochemie Moleculaire Biologie Geneeskunde Kankerbiologie Biofysica Chemische Biologie Proteïnen microscopie fluorescentie autofagie arginine deiminase prostaatkanker deconvolutie microscopie super-resolutie gestructureerde-verlichting microscopie live cell imaging tumoren autofagosomen lysosomen cellen celcultuur microscopie imaging visualisatie
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