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Samsvarende Mikroskopi for 3D Structural Analysis of Dynamic Interaksjoner

Article DOI: 10.3791/50386-v • 13:43 min • June 24th, 2013
June 24th, 2013 •
1, 1, 1, 2, 2, 1
1Department of Structural Biology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

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Vi beskriver en samsvarende mikroskopi metode som kombinerer høyhastighets 3D live-cell fluorescerende lys mikroskopi og høy oppløsning Cryo-elektron tomografi. Vi demonstrerer evnen til correlative metoden ved bildebehandling dynamisk, små HIV-1 partikler i samspill med host Jakten celler.

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Bioteknologi Molecular Biology Structural Biology virologi biofysikk cellebiologi fysiologi medisin Biomedical Engineering Infeksjon mikrobiologi teknologi industri jordbruk miljø-og biovitenskap (General) samsvarende mikroskopi CryoET Cryo-elektron tomografi Confocal live-cell imaging Cryo-fluorescens lysmikroskopi HIV-1 capsid HeLa celle virus mikroskopi bildebehandling
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