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Immunology and Infection

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JoVE Journal Immunology and Infection
Hoogst vastbesloten Intravitale Striped-verlichting Microscopie van Germinal Centers
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Hoogst vastbesloten Intravitale Striped-verlichting Microscopie van Germinal Centers

Article DOI: 10.3791/51135-v • 10:07 min • April 9th, 2014
April 9th, 2014 •
*1,2, *3, 4, 2, *3,5, *1
1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine
* These authors contributed equally

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Hoge-resolutie intravitale beeldvorming met verbeterd contrast tot 120 urn diepte lymfklieren van volwassen muizen wordt bereikt door ruimtelijk moduleren van de excitatie patroon van een multi-focale twee-foton microscoop. In 100 micrometer diepte gemeten we resoluties van 487 nm (lateraal) en 551 nm (axiaal), waardoor verstrooiing en diffractie grenzen omzeilen.

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Immunologie twee-foton laser scanning microscopie deep-tissue intravitale beeldvorming kiemcentrum lymfeklieren hoge-resolutie een verbeterd contrast
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