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DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Article DOI: 10.3791/51715-v • 12:24 min • July 21st, 2014
July 21st, 2014 •
1, 1, 1
1Physical Biosciences Division, Lawrence Berkeley National Laboratory

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This video article describes an in vitro microarray based method to determine the gene targets and binding sites for two component system response regulators.

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DNA-affinity-purified Chip (DAP-chip) Gene Targets Bacterial Two Component Regulatory Systems ChIP-chip In Vivo Methods Transcription Factors Activation Conditions In Vitro Microarray Response Regulators Binding Sites Sequenced Genome Tagged Protein Sheared Genomic DNA Affinity Purification Fluorescent Labeling Custom Tiling Array Assay Optimization Array Data Analysis Binding Site Motifs Experimentally Validated Orthologous Response Regulators
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