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Odling

Article DOI: 10.3791/51796-v • 08:26 min • August 2nd, 2014
August 2nd, 2014 •
1, 1, 1, 1,2
1Department of Animal and Avian Sciences, University of Maryland, 2Department of Cell Biology and Molecular Genetics, University of Maryland

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C. elegans är oftast odlas på fasta agarplattor eller i flytande kulturer ympats med E. coli. För att förhindra bakteriella biprodukter från confounding toxikologiska och näringsmässiga undersökningar, utnyttjade vi en axenisk flytande medium, CeHR, att växa och synkronisera ett stort antal maskar för en rad tillämpningar i efterföljande led.

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Molecular Biology , axeniska medier transgena mikropartikelbeskjutning heme näringslära
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