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SILAC Based proteomik karakterisering av exosomes från HIV-1-infekterade celler

Article DOI: 10.3791/54799-v • 10:24 min • March 3rd, 2017
March 3rd, 2017 •
1, 1, 1, 2,3, 3
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University

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Här beskriver vi en kvantitativ proteomik metod som använder tekniken med stabila isotopen märkning av aminosyror i cellkultur (SILAC) att analysera effekterna av HIV-1-infektion på värd exosomal proteom. Detta protokoll kan enkelt anpassas till celler under olika stress eller infektionsförhållanden.

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Infektion exosomes extracellulära Fordon HIV-1 proteomik masspektrometri SILAC bioinformatik
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