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JoVE Journal Developmental Biology
Organ kultur och Hela Mount immunofluorescensfärgning av Mus Wolffian kanaler
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Organ kultur och Hela Mount immunofluorescensfärgning av Mus Wolffian kanaler

Article DOI: 10.3791/55134-v • 09:16 min • January 13th, 2017
January 13th, 2017 •
1, 1
1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

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Vi presenterar en metod för isolering och odling av mus Wolffian kanalen (WD). Vi visar också en detaljerad beskrivning av hela berget immunfärgning av odlade / nyligen isolerade WDS med fluorescerande taggade antikroppar. Tillsammans utgör dessa tekniker möjliggör studier av WD utveckling, ringlande och differentiering.

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Utvecklingsbiologi epididymis organkultur immunfärgning Wolffian kanal hela berget,
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