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Generatie Fluorescent Protein Fusies

Article DOI: 10.3791/55333-v • 09:27 min • March 4th, 2017
March 4th, 2017 •
1, 2, 1
1Department of Pediatrics, University of Minnesota, 2Department of Molecular Microbiology and Biotechnology, Tel Aviv University

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PCR-gemedieerde genetische modificatie kan worden gebruikt voor fluorescent proteïne fusies in Candida soorten die visualisatie en kwantificering van gistcellen en eiwitten vergemakkelijkt genereren. Hierin presenteren we een strategie voor het construeren van een fluorescerend eiwit fusie (Eno1-FP) Candida parapsilosis.

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Genetics , PCR-gemedieerde genetische modificatie Fluorescent proteïne, Lithium-acetaat transformatie,
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