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Journal / Biochemistry / 单分子水平的高精度FRET用于生物分子结构测定…
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Biochemistry

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JoVE Journal Biochemistry
单分子水平的高精度FRET用于生物分子结构测定
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单分子水平的高精度FRET用于生物分子结构测定

Article DOI: 10.3791/55623-v • 11:24 min • May 13th, 2017
May 13th, 2017 •
1, 2, 2, 3, 3, 2
1Department of Chemistry, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences, University of Texas Health Science Center

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本文介绍了单分子水平高精度FRET实验方案。此外，该方法可用于鉴定N-甲基-D-天冬氨酸（NMDA）受体的配体结合结构域中的三种构象状态。确定精确的距离是建立基于FRET实验的结构模型的第一步。

Tags

生物化学，第123期，FRET，单分子，间隔距离，DNA，荧光团，多参数荧光检测，脉冲交错激发，蛋白质标记
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