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Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
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Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

Article DOI: 10.3791/55797-v • 11:00 min • September 18th, 2017
September 18th, 2017 •
*1, *2, 1, 1, 3, 1, 1
1Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 2Experimental Research Center, China Academy of Chinese Medical Sciences, 3Department of Cardiology, The Third Hospital of Hangzhou City
* These authors contributed equally

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Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Here, we describe a protocol to evaluate sarcoplasmic reticulum Ca2+ reserve and diastolic calcium removal function in cardiomyocytes by a calcium imaging system.

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Sarcoplasmic Reticulum Calcium Reserve Intracellular Diastolic Calcium Removal Isolated Ventricular Cardiomyocytes Calcium Handling Mechanism Pathophysiological Conditions Langendorff Perfusion System NT Solution Temperature Abdominal Cavity Xiphoid Process Chest Opening Pericardium Removal Aortic Arch Identification Heart Removal Dish Washing Cannula Mounting Aorta Ligation Perfusion Process
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