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JoVE Journal Developmental Biology
Isolering av humane myoblaster, vurdering av myogen differensiering og oppbevaringsoperert kalsiummåling
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Isolering av humane myoblaster, vurdering av myogen differensiering og oppbevaringsoperert kalsiummåling

Article DOI: 10.3791/55918-v • 10:45 min • July 26th, 2017
July 26th, 2017 •
*1, *2, 2,3, 3
1Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences, Geneva Medical Center, 3Department of Cell Physiology and Metabolism, Geneva Medical Center
* These authors contributed equally

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Her beskriver vi en metode for å oppnå en ren populasjon av humane myoblaster fra voksen muskelvev. Disse cellene brukes til å studere in vitro skjelettmuskeldifferensiering og spesielt å studere proteiner involvert i Ca 2+ -signalering.

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Utviklingsbiologi utgave 125 myogene celler myoblastdifferensiering Ca butikkoperert Ca markører for differensiering fluorescensaktivert cellesortering
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