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In Vivo Detektion und Analyse von Rb Protein Sumoylierung in menschlichen Zellen

Article DOI: 10.3791/56096-v • 09:41 min • November 2nd, 2017
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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Kleine Ubiquitin-bezogene Modifikator (SUMO) Familie Proteine sind an Lysin-Reste von Zielproteine, verschiedene zelluläre Prozesse regulieren konjugiert. Dieses Whitepaper beschreibt ein Protokoll für den Nachweis von Retinoblastom (Rb) Protein Sumoylierung endogene und exogene Bedingungen in menschlichen Zellen.

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Molekulare Biologie Ausgabe 129 Retinoblastoma Protein (Rb) Post-translationale Modifikation (PTM) kleine Ubiquitin-bezogene Modifikator (SUMO) Zellzyklus HKE293 Zellen
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