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Journal / Neuroscience / 应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞…
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Neuroscience

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JoVE Journal Neuroscience
应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞
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应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞

Article DOI: 10.3791/56538-v • 12:34 min • December 6th, 2017
December 6th, 2017 •
1, 1,2,3, 1,3
1Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, 2Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, 3German Center for Diabetes Research (DZD)

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由于组织的缺乏, 小鼠坐骨神经内细胞含量的定量分析是困难的。本协议描述了一种组织消化和制备的方法, 它提供了足够的细胞, 用于对单个小鼠神经的免疫细胞进行流式细胞仪分析。

Tags

神经科学 问题 130 流式细胞仪 小鼠 坐骨神经 巨噬细胞 炎症 白细胞 神经病
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