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Journal / Chemistry / 用于化学诱导的 DNA 化和表观遗传重塑的基因工程 Split-TET2…
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JoVE Journal Chemistry
用于化学诱导的 DNA 化和表观遗传重塑的基因工程 Split-TET2 酶
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用于化学诱导的 DNA 化和表观遗传重塑的基因工程 Split-TET2 酶

Article DOI: 10.3791/56858-v • 08:34 min • December 18th, 2017
December 18th, 2017 •
1, 2, 1
1Centre for Epigenetics and Disease Prevention, Department of Molecular and Cellular Medicine, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2Centre for Translational Cancer Research, Department of Medical Physiology, Institute of Biosciences and Technology, College of Medicine, Texas A&M University

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介绍了将工程化的 split-TET2 酶 (苹果酒) 引入哺乳动物细胞中用于化学诱导 DNA 化和后生重塑的详细协议。

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化学 问题 130 dna 甲基化 胚胎遗传学 染色质可及性 dna 甲基 化学生物学 TET2 5-hydroxymethylcytosine 转录 基因表达 基因编辑
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