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En manipuleret Split-TET2 enzym til kemikalie-inducerbar DNA Hydroxymethylation og epigenetiske Remodeling
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En manipuleret Split-TET2 enzym til kemikalie-inducerbar DNA Hydroxymethylation og epigenetiske Remodeling

Article DOI: 10.3791/56858-v • 08:34 min • December 18th, 2017
December 18th, 2017 •
1, 2, 1
1Centre for Epigenetics and Disease Prevention, Department of Molecular and Cellular Medicine, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2Centre for Translational Cancer Research, Department of Medical Physiology, Institute of Biosciences and Technology, College of Medicine, Texas A&M University

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Detaljerede protokoller for at indføre et manipuleret split-TET2 enzym (CiDER) i pattedyrceller for kemiske inducerbar DNA hydroxymethylation og epigenetiske remodeling præsenteres.

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Kemi spørgsmålet 130 DNA methylering Epigenetik kromatin tilgængelighed DNA demetylering kemisk biologi TET2 5-hydroxymethylcytosine transskription Gen-ekspression epigenome redigering
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