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An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
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An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Article DOI: 10.3791/56858-v • 08:34 min • December 18th, 2017
December 18th, 2017 •
1, 2, 1
1Centre for Epigenetics and Disease Prevention, Department of Molecular and Cellular Medicine, Institute of Biosciences and Technology, College of Medicine, Texas A&M University, 2Centre for Translational Cancer Research, Department of Medical Physiology, Institute of Biosciences and Technology, College of Medicine, Texas A&M University

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Detailed protocols for introducing an engineered split-TET2 enzyme (CiDER) into mammalian cells for chemical inducible DNA hydroxymethylation and epigenetic remodeling are presented.

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Engineered Split-TET2 Enzyme CiDER DNA Hydroxymethylation Epigenetic Remodeling Mammalian Cells Temporal Control 5-methylcytosine Oxidation Epigenetic States Chemical Additives Culture DMEM Supplemented With 10% Heat-inactivated FBS Penicillin And Streptomycin Transfection Rapamycin Induction Flow Cytometry FACS Buffer Control Group
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