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Imagerie de cellules vivantes de la ségrégation des chromosomes pendant la mitose

Article DOI: 10.3791/57389-v • 06:39 min • March 14th, 2018
March 14th, 2018 •
1, 2, 1
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration

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Ce protocole décrit une méthode simple et pratique pour étiqueter et visualiser direct chromosomes dans les cellules mitotiques en utilisant l’étiquette Histone2B-GFP BacMam 2.0 et un système de microscopie confocale tourne disque.

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Numéro 133 ségrégation des chromosomes contrôle du cycle cellulaire checkpoint Assemblée broche biologie du développement des cellules vivantes imagerie la nocodazole fibroblastes embryonnaires de souris protéine Phosphatase 2 a Histone2B-GFP BacMam 2.0
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