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Laser fange Microdissection av svært ren trabekulært Meshwork fra musen øyne for Gene Expression analyse

Article DOI: 10.3791/57576-v • 13:47 min • June 3rd, 2018
June 3rd, 2018 •
*1, *2, *1, 2, 2, 2,3, 2,4, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
* These authors contributed equally

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Her beskriver vi en reproduserbar laser fange microdissection (LCM) er en protokoll for å isolere trabekulært meshwork (TM) for nedstrøms RNA analyse. Muligheten til å analysere endringer i byggkorn under TM vil hjelpe forstå underliggende molekylære mekanismer av TM-relaterte okulær sykdommer.

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Biologi problemet 136 grønn stær trabekulært meshwork laser fange microdissection laser microdissection RNA isolasjon genekspresjon LCM LMD
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