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Biochemistry

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JoVE Journal Biochemistry
Kalibrering-fri In Vitro kvantifisering av Protein Homo-oligomerization med kommersielle instrumentering og gratis, åpen kildekode lysstyrke analyseprogramvare
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Journal (Biochemistry)
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
 
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Kalibrering-fri In Vitro kvantifisering av Protein Homo-oligomerization med kommersielle instrumentering og gratis, åpen kildekode lysstyrke analyseprogramvare

Article DOI: 10.3791/58157-v • 08:22 min • July 17th, 2018
July 17th, 2018 •
1, 1, 2, 2, 2, 1,2,3,4
1Cellular Imaging Group, Wellcome Centre Human Genetics, University of Oxford, 2Division of Structural Biology, Wellcome Centre Human Genetics, University of Oxford, 3Dynamic Structural Virology Group, Biocruces Health Research Centre, 4IKERBASQUE, Basque Foundation for Science

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Denne protokollen beskriver en kalibrering-fri tilnærming for å kvantifisere protein homo-oligomerization i vitro basert på fluorescens svingninger spektroskopi med kommersielle lyset skanning mikroskopi. Riktig oppkjøpet innstillingene og analyseteknologi vises.

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Biokjemi problemet 137 Dimerization kalibrering gratis spektroskopi fluorescens svingninger nummer og lysstyrken protein aggregering ett molekyl mikroskopi
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