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Immunology and Infection

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JoVE Journal Immunology and Infection
Måling af Dengue Virus RNA i cellekultur Supernatant af inficerede celler ved Real-time kvantitativ Polymerase Chain Reaction
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Måling af Dengue Virus RNA i cellekultur Supernatant af inficerede celler ved Real-time kvantitativ Polymerase Chain Reaction

Article DOI: 10.3791/58407-v • 08:36 min • November 1st, 2018
November 1st, 2018 •
1, 1, 1, 1, 1, 1
1Department of Microbiology and Infection Control, Osaka Medical College

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Real-time kvantitativ polymerase kædereaktion analyse kombineret med reverse transkription (RT-qPCR) har været meget anvendt til at måle niveauet af RNA-virusinfektioner. Her præsenterer vi en direkte RT-qPCR assay, som ikke kræver en RNA oprensning skridt, udviklet til kvantificering af flere RNA-vira, herunder dengue virus.

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Immunologi og infektion sag 141 direkte RT-qPCR assay viral RNA påvisning kultur supernatanten ingen RNA oprensning skridt dengue virus gul feber virus Chikungunya virus mæslingevirus
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